Human RALBP1 Associated Eps Domain Containing Protein 1 (REPS1) ELISA Kit KN-E0149H

Regulatory Status:

RUO

Alternative:

RalBP Associated Eps15-Homology Domain protein; RalBP1-interacting protein 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.082ng/mL

Specificity:

This kit recognizes Human REPS1 in samples. No significant cross-reactivity or interference between Human REPS1 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| REPS1

RalBP1-associated Eps domain-containing protein 1 is a protein that is encoded by the REPS1 gene.This gene encodes a signaling adaptor protein with two EH domains that interacts with Proteins that participate in signaling, endocytosis and cytoskeletal changes. The encoded protein has been found in association with intersectin 1 and Src homology 3-domain growth factor receptor-bound 2-like (endophilin) interacting protein 1 when intersectin 1 was isolated from clathrin-coated pits. The encoded protein has also been shown to interact with amphiphysin, a cytoplasmic protein at the surface of synaptic vesicles. Alternative splicing results in multiple transcript variants encoding different isoforms.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human REPS1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human REPS1 and diluted with Universal Diluent to produce samples with values within the range of the assay.