Human Squamous Cell Carcinoma Antigen 2 (SCCA2) ELISA Kit KB-E0273H

Alternative:

SERPINB4; PI11; SPB4; Leupin; Serpin Peptidase Inhibitor,Clade B,Member 4; Peptidase Inhibitor 11

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.06ng/mL

Specificity:

This kit recognizes Human SCCA2 in samples. No significant cross-reactivity or interference between Human SCCA2 and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Cancer

| SCCA2

Serpin B4 is a protein that is encoded by the SERPINB4 gene.The protein encoded by this gene is a member of the serpin family of serine protease inhibitors. The encoded protein is highly expressed in many tumor cells and can inactivate granzyme M, an enzyme that kills tumor cells. This protein, along with serpin B3, can be processed into smaller fragments that aggregate to form an autoantigen in psoriasis, probably by causing chronic inflammation.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human SCCA2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human SCCA2 and diluted with Universal Diluent to produce samples with values within the range of the assay.