Rat Integrin Alpha M (CD11b) ELISA Kit KB-E21040R

Regulatory Status:

RUO

Alternative:

ITGaM; ITG-AM; CR3A; MAC-1; MAC1A; MO1A; Complement Component 3 Receptor 3 Subunit; Cell surface glycoprotein MAC-1 subunit alpha; Neutrophil adherence receptor

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.31-20ng/mL

Sensitivity:

0.16ng/mL

Specificity:

This kit recognizes Rat CD11b in samples. No significant cross-reactivity or interference between Rat CD11b and analogues was observed.

Sample Type:

tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| CD11b

Integrin alpha M (ITGAM) is one protein subunit that forms heterodimeric integrin alpha-M beta-2 (αMβ2) molecule, also known as macrophage-1 antigen (Mac-1) or complement receptor 3 (CR3).ITGAM is also known as CR3A, and cluster of differentiation molecule 11B (CD11B). The second chain of αMβ2 is the common integrin β2 subunit known as CD18, and integrin αMβ2 thus belongs to the β2 subfamily (or leukocyte) integrins.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat CD11b spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Rat CD11b and diluted with Universal Diluent to produce samples with values within the range of the assay.