Human Complement C3 Convertase (C3 Convertase) ELISA Kit KB-E1294H

Regulatory Status:

RUO

Alternative:

C4b2a; C3bBb, complement C.hivin.4.hivin2

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.78-50ng/mL

Sensitivity:

0.32ng/mL

Specificity:

This kit recognizes Human C3 Convertase in samples. No significant cross-reactivity or interference between Human C3 Convertase and analogues was observed.

Sample Type:

serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Signal Transduction

| C3 Convertase

C3 convertase (C4bC2b, formerly C4b2a) belongs to family of serine proteases and is necessary in innate immunity as a part of the complement system which eventuate in opsonisation of particles, release of inflammatory peptides, C5 convertase formation and cell lysis.C3 convertase can be used to refer to the form produced in the alternative pathway (C3bBb) or the classical and lectin pathways (C4bC2b, formerly C4b2a). Once formed, both C3 convertases will catalyze the proteolytic cleavage of C3 into C3a and C3b (hence the name "C3-convertase").

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human C3 Convertase spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human C3 Convertase and diluted with Universal Diluent to produce samples with values within the range of the assay.