Rat Caveolin 1 (CAV1) ELISA Kit KB-E23627R

Regulatory Status:

RUO

Alternative:

VIP21; Caveolae Protein 22kDa

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.08ng/mL

Specificity:

This kit recognizes Rat CAV1 in samples. No significant cross-reactivity or interference between Rat CAV1 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Cancer

 CAV1

Caveolin-1 is a protein that is encoded by the CAV1 gene.The scaffolding protein encoded by this gene is the main component of the caveolae plasma membranes found in most cell types. The protein links integrin subunits to the tyrosine kinase FYN, an initiating step in coupling integrins to the Ras-ERK pathway and promoting cell cycle progression. The gene is a tumor suppressor gene candidate and a negative regulator of the Ras-p42/44 MAP kinase cascade. CAV1 and CAV2 are located next to each other on chromosome 7 and express colocalizing Proteins that form a stable hetero-oligomeric complex.By using alternative initiation codons in the same reading frame, two isoforms (alpha and beta) are encoded by a single transcript from this gene.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

 Recovery

The recovery of Rat CAV1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Linearity

Samples were spiked with high concentrations of Rat CAV1 and diluted with Universal Diluent to produce samples with values within the range of the assay.