Mouse Crk Like Protein (CRKL) ELISA Kit KB-E13466M

Regulatory Status:

RUO

Alternative:

V-Crk Sarcoma Virus CT10 Oncogene Homolog(avian)-Like

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78.12-5000pg/mL

Sensitivity:

39pg/mL

Specificity:

This kit recognizes Mouse CRKL in samples. No significant cross-reactivity or interference between Mouse CRKL and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Cancer

CRKL

Crk-like protein is a protein that is encoded by the CRKL gene.v-CRK avian sarcoma virus CT10-homolog-like contains one SH2 domain and two SH3 domains. CRKL has been shown to activate the RAS and JUN kinase signaling pathways and transform fibroblasts in a RAS-dependent fashion. It is a substrate of the BCR-ABL tyrosine kinase and plays a role in fibroblast transformation by BCR-ABL. In addition, CRKL has oncogenic potential.CrkL together with Crk participates in the Reelin signaling cascade downstream of DAB1.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

Recovery

The recovery of Mouse CRKL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Linearity

Samples were spiked with high concentrations of Mouse CRKL and diluted with Universal Diluent to produce samples with values within the range of the assay.