Human Receptor-type tyrosine-protein phosphatase zeta (PTPRZ) ELISA Kit KB-E2936H

Regulatory Status:RUO

Alternative:PTPRZ1; HPTPZ; HPTPzeta; PTP18; PTPR-Z; PTPZ; RPTPB; RPTPbeta; Phosphacan; Protein Tyrosine Phosphatase Receptor Type Z

Regulatory Status:

RUO

Alternative:

PTPRZ1; HPTPZ; HPTPzeta; PTP18; PTPR-Z; PTPZ; RPTPB; RPTPbeta; Phosphacan; Protein Tyrosine Phosphatase Receptor Type Z

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.07ng/mL

Specificity:

This kit recognizes Human PTPRZ in samples. No significant cross-reactivity or interference between Human PTPRZ and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction


| PTPRZ


Receptor-type tyrosine-protein phosphatase zeta also known as phosphacan is an enzyme that is encoded by the PTPRZ1 gene.This gene is a member of the receptor tyrosine phosphatase family and encodes a single-pass type I membrane protein with two cytoplasmic tyrosine-protein phosphatase domains, an alpha-carbonic anhydrase domain and a fibronectin type III domain. Alternative splice variants that encode different protein isoforms have been described but their full-length nature has not been determined.

 

| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


  

Concentration (ng/mL)1052.51.250.6250.3120.1560
OD2.041.40.890.640.420.270.210.06
Corrected OD1.981.340.830.580.360.210.15-

 






| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Human PTPRZ spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


 

Sample TypeRange(%)Average Recovery(%)
Tissue Homogenates(n=8)85-10196
Cell Lysates(n=8)92-105103






 

| Linearity


Samples were spiked with high concentrations of Human PTPRZ and diluted with Universal Diluent to produce samples with values within the range of the assay.


 



Tissue Homogenates(n=4)Cell Lysates(n=4)
1 : 2Range (%)84-9586-96
Average (%)9193
1 : 4Range (%)89-10387-108
Average (%)9598








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