Human solubility AXL Receptor Tyrosine Kinase (sAXL) ELISA Kit KB-E2154H

Regulatory Status:

RUO

Alternative:

sAXL

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.16ng/mL

Specificity:

This kit recognizes Human sAXL in samples. No significant cross-reactivity or interference between Human sAXL and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates and other biological fluids

Research Area:

Cancer

sAXL

Tyrosine-protein kinase receptor UFO is a protein that is encoded by the AXL gene. The gene was initially designated as UFO, in allusion to the unidentified function of this protein.However, in the years since its discovery, research into AXL's expression profile and mechanism has made it an increasingly attractive target, especially for cancer therapeutics. In recent years, AXL has emerged as a key facilitator of immune escape and drug-resistance by cancer cells, leading to aggressive and metastatic cancers.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

Recovery

The recovery of Human sAXL spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Linearity

Samples were spiked with high concentrations of Human sAXL and diluted with Universal Diluent to produce samples with values within the range of the assay.