Regulatory Status:RUO
Alternative:RBM8A; BOV-1A; BOV-1B; BOV-1C; RBM8B; Y14; ZNRP; ZRNP1; Binder of OVCA1-1; RNA-binding protein Y14; Ribonucleoprotein RBM8A;RNA Binding Motif Protein 8
Regulatory Status:
Alternative:
Detection Method:
ELISA
Assay Type:
Sandwich
Detection Range:
0.156-10ng/mL
Sensitivity:
0.08ng/mL
Specificity:
This kit recognizes Human RBM8 in samples. No significant cross-reactivity or interference between Human RBM8 and analogues was observed.
Sample Type:
Tissue homogenates, cell lysates and other biological fluids
Research Area:
Signal Transduction
RNA-binding protein 8A is a protein that is encoded by the RBM8A gene.This gene encodes a protein with a conserved RNA-binding motif. The protein is found predominantly in the nucleus, although it is also present in the cytoplasm. It is preferentially associated with mRNAs produced by splicing, including both nuclear mRNAs and newly exported cytoplasmic mRNAs.
As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.
Concentration (ng/mL) | 10 | 5 | 2.5 | 1.25 | 0.625 | 0.312 | 0.156 | 0 |
OD | 2.03 | 1.21 | 0.69 | 0.41 | 0.29 | 0.23 | 0.19 | 0.09 |
Corrected OD | 1.94 | 1.12 | 0.6 | 0.32 | 0.2 | 0.14 | 0.1 | - |
Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%
The recovery of Human RBM8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.
Sample Type | Range(%) | Average Recovery(%) |
Tissue Homogenates(n=8) | 82-101 | 96 |
Cell Lysates(n=8) | 92-105 | 103 |
Samples were spiked with high concentrations of Human RBM8 and diluted with Universal Diluent to produce samples with values within the range of the assay.
Tissue Homogenates(n=4) | Cell Lysates(n=4) | ||
1 : 2 | Range (%) | 84-95 | 86-96 |
Average (%) | 91 | 93 | |
1 : 4 | Range (%) | 89-103 | 87-108 |
Average (%) | 95 | 98 |
CATALOGUE