Human Transcription factor E2F1 (E2F1) ELISA Kit KB-E0271H

Regulatory Status:

RUO

Alternative:

RBBP3; RBP3; RBAP-1; Retinoblastoma-associated protein 1; Retinoblastoma-binding protein 3; pRB-binding protein E2F-1;E2F Transcription Factor 1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.07ng/mL

Specificity:

This kit recognizes Human E2F1 in samples. No significant cross-reactivity or interference between Human E2F1 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| E2F1

Transcription factor E2F1 is a protein that is encoded by the E2F1 gene.The protein encoded by this gene is a member of the E2F family of transcription factors. The E2F family plays a crucial role in the control of cell cycle and action of tumor suppressor Proteins and is also a target of the transforming Proteins of small DNA tumor viruses. The E2F Proteins contain several evolutionarily conserved domains found in most members of the family. This protein and another 2 members, E2F2 and E2F3, have an additional cyclin binding domain. This protein binds preferentially to retinoblastoma protein pRB in a cell-cycle dependent manner. It can mediate both cell proliferation and p53-dependent/independent apoptosis.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human E2F1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human E2F1 and diluted with Universal Diluent to produce samples with values within the range of the assay.