Human Retinoic acid-induced protein 3 (GPRC5A) ELISA Kit KB-E3179H

Regulatory Status:

RUO

Alternative:

RAIG1; RAI3, GPCR5A; G Protein Coupled Receptor, Family C, Group 5, Member A; Orphan G-protein-coupling receptor PEIG-1; Retinoic acid-induced gene 1 protein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.312-20ng/mL

Sensitivity:

0.153ng/mL

Specificity:

This kit recognizes Human GPRC5A in samples. No significant cross-reactivity or interference between Human GPRC5A and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids.

Research Area:

Signal Transduction

| GPRC5A

Retinoic acid-induced protein 3 is a protein that is encoded by the GPRC5A gene.This gene and its encoded mRNA was first identified as a phorbol ester-induced gene, and named Phorbol Ester Induced Gen 1 (PEIG-1); two years later it was rediscovered as a retinoic acid-inducible gene, and named Retinoic Acid-Inducible Gene 1 (RAIG1). Its encoded protein was later named Retinoic acid-induced protein 3.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human GPRC5A spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human GPRC5A and diluted with Universal Diluent to produce samples with values within the range of the assay.