Human Uroplakin 2 (UPK2) ELISA Kit KB-E2199H

Regulatory Status:

RUO

Alternative:

UPII; Uroplakin II

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.072ng/mL

Specificity:

This kit recognizes Human UPK2 in samples. No significant cross-reactivity or interference between Human UPK2 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| UPK2

Uroplakin-2 (UP2) is a protein that is encoded by the UPK2 gene.This gene encodes one of the Proteins of the highly conserved urothelium-specific integral membrane Proteins of the asymmetric unit membrane which forms urothelium apical plaques in mammals. The asymmetric unit membrane is believed to strengthen the urothelium by preventing cell rupture during bladder distention. The encoded protein is expressed in the peripheral blood of bladder cancer patients with transitional cell carcinomas.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human UPK2 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human UPK2 and diluted with Universal Diluent to produce samples with values within the range of the assay.