Product Name:

Rat Matrix Metalloproteinase 8 (MMP-8) ELISA Kit

Regulatory Status:

RUO

Alternative:

CLG2; HNC; PMNL-CL; Collagenase 2; Neutrophil Collagenase; PMNL collagenase

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.62-40ng/mL

Sensitivity:

0.26ng/mL

Specificity:

This kit recognizes Rat MMP-8 in samples. No significant cross-reactivity or interference between Rat MMP-8 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

MMP-8

Neutrophil collagenase, also known as matrix metalloproteinase-8 (MMP-8) or PMNL collagenase (MNL-CL), is a collagen cleaving enzyme which is present in the connective tissue of most mammals.The MMP-8 protein is encoded by the MMP8 gene.The gene is part of a cluster of MMP genes which localize to chromosome 11q22.3. Most MMP's are secreted as inactive proProteins which are activated when cleaved by extracellular proteinases. However, the enzyme encoded by this gene is stored in secondary granules within neutrophils and is activated by autolytic cleavage.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat MMP-8 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Rat MMP-8 and diluted with Universal Diluent to produce samples with values within the range of the assay.