Human Fibulin 1 (FBLN1) ELISA Kit KB-E3087H

Regulatory Status:

RUO

Alternative:

FBLN1

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

3.12-200ng/mL

Sensitivity:

1.6ng/mL

Specificity:

This kit recognizes Human FBLN1 in samples. No significant cross-reactivity or interference between Human FBLN1 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Cancer

FBLN1

FBLN1 is the gene encoding fibulin-1, an extracellular matrix and plasma protein.Fibulin-1 is a secreted glycoprotein that is found in association with extracellular matrix structures including fibronectin-containing fibers, elastin-containing fibers and basement membranes. Fibulin-1 binds to a number of extracellular matrix constituents including fibronectin,nidogen-1, and the proteoglycan, versican. Fibulin-1 is also a blood protein capable of binding to fibrinogen.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

Recovery

The recovery of Human FBLN1 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Linearity

Samples were spiked with high concentrations of Human FBLN1 and diluted with Universal Diluent to produce samples with values within the range of the assay.