Human Adenosine A2a Receptor (ADORA2a) ELISA Kit KB-E3198H

Regulatory Status:

RUO

Alternative:

ADORA2; RDC8; hA2AR

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.15-10ng/mL

Sensitivity:

0.1ng/mL

Specificity:

This kit recognizes Human ADORA2a in samples. No significant cross-reactivity or interference between Human ADORA2a and analogues was observed.

Sample Type:

Tissue homogenates and other biological fluids

Research Area:

Cancer

ADORA2a

The adenosine A2A receptor, also known as ADORA2A, is an adenosine receptor.This protein is a member of the G protein-coupled receptor (GPCR) family which possess seven transmembrane alpha helices, as well as an extracellular N-terminus and an intracellular C-terminus. Furthermore, located in the intracellular side close to the membrane is a small alpha helix, often referred to as helix 8 (H8). The crystallographic structure of the adenosine A2A receptor reveals a ligand binding pocket distinct from that of other structurally determined GPCRs (i.e., the beta-2 adrenergic receptor and rhodopsin).
 

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

Recovery

The recovery of Human ADORA2a spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 Linearity

Samples were spiked with high concentrations of Human ADORA2a and diluted with Universal Diluent to produce samples with values within the range of the assay.