Human Retinoschisin (RS) ELISA Kit KB-E2553H

Regulatory Status:

RUO

Alternative:

RS1; XLRS1; Retinoschisis X-Linked,Juvenile 1; X-linked juvenile retinoschisis protein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.08ng/mL

Specificity:

This kit recognizes Human RS in samples. No significant cross-reactivity or interference between Human RS and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| RS

Retinoschisin also known as X-linked juvenile retinoschisis protein is a lectin that is encoded by the RS1 gene.It is a soluble, cell-surface protein that plays an important role in the maintenance of the retina where it is expressed and secreted by retinal bipolar cells and photoreceptors,[8][9] as well as in the pineal gland.[10] Retinoschisin (RS1) is encoded by the gene RS1 located on the X chromosome at p22.1. Young males who have an RS1 mutation are susceptible to retinoschisis, and X-linked eye disease which causes macular degeneration and can lead to a loss of vision.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Human RS spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Human RS and diluted with Universal Diluent to produce samples with values within the range of the assay.