Mouse Wilson disease protein (WND) ELISA Kit KB-E3515M

Regulatory Status:

RUO

Alternative:

PWD; WC1; WD; ATP7b; ATPase, Cu++ Transporting Beta Polypeptide; Copper pump 2; Wilson disease-associated protein

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.081ng/mL

Specificity:

This kit recognizes Mouse WND in samples. No significant cross-reactivity or interference between Mouse WND and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction

| WND

Wilson disease protein (WND), also known as ATP7B protein, is a copper-transporting P-type ATPase which is encoded by the ATP7B gene. The ATP7B protein is located in the trans-Golgi network of the liver and brain and balances the copper level in the body by excreting excess copper into bile and plasma. Genetic disorder of the ATP7B gene may cause Wilson's disease, a disease in which copper accumulates in tissues, leading to neurological or psychiatric issues and liver diseases.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Mouse WND spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Mouse WND and diluted with Universal Diluent to produce samples with values within the range of the assay.