Product Name:

Human Tumor necrosis factor-inducible gene 6 protein (TNFαIP6) ELISA Kit

Regulatory Status:

RUO

Alternative:

TSG6; TNF-Stimulated Gene 6; Hyaluronate-binding protein; Tumor necrosis factor alpha-induced protein 6

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

15.6-1000pg/mL

Sensitivity:

7.2pg/mL

Specificity:

This kit recognizes Human TNFαIP6 in samples. No significant cross-reactivity or interference between Human TNFαIP6 and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

Research Area:

Cancer

TNFαIP6

Tumor necrosis factor-inducible gene 6 protein also known as TNF-stimulated gene 6 protein or TSG-6 is a protein that is encoded by the TNFAIP6 (tumor necrosis factor, alpha-induced protein 6) gene.TSG-6 is a 30 kDa secreted protein that contains a hyaluronan-binding LINK domain a and thus is a member of the hyaluronan-binding protein family, also called hyaladherins. The hyaluronan-binding domain is known to be involved in extracellular matrix stability and cell migration. This protein has been shown to form a stable, covalent complex with inter-alpha-inhibitor (IαI), and thus enhance the serine protease inhibitory activity of IαI, which is important in the protease network associated with inflammation.

Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

Recovery

The recovery of Human TNFαIP6 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

Linearity

Samples were spiked with high concentrations of Human TNFαIP6 and diluted with Universal Diluent to produce samples with values within the range of the assay.