Product Name:

Rat Plasminogen Activator, Urokinase (uPA) ELISA Kit

Regulatory Status:

RUO

Alternative:

PLAU; ATF; URK; UK; UP-A; Abbokinase; Urokinase-Type Plasminogen Activator；Plasminogen Activator, Urokinase

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

15.62-1000pg/mL

Sensitivity:

5.72pg/mL

Specificity:

This kit recognizes Rat uPA in samples. No significant cross-reactivity or interference between Rat uPA and analogues was observed.

Sample Type:

Serum, plasma, tissue homogenates, cell lysates, cell culture supernates and other biological fluids

| uPA

Urokinase, also known as urokinasetype plasminogen activator (uPA), is a serine protease present in humans and other animals. The human urokinase protein was discovered, but not named, by McFarlane and Pilling in 1947[5] Urokinase was originally isolated from human urine, and it is also present in the blood and in the extracellular matrix of many tissues. The primary physiological substrate of this enzyme is plasminogen, which is an inactive form (zymogen) of the serine protease plasmin. Activation of plasmin triggers a proteolytic cascade that, depending on the physiological environment, participates in thrombolysis or extracellular matrix degradation. This cascade had been involved in vascular diseases and cancer progression.

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%

| Recovery

The recovery of Rat uPA spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

| Linearity

Samples were spiked with high concentrations of Rat uPA and diluted with Universal Diluent to produce samples with values within the range of the assay.