Human Family With Sequence Similarity 19, Member A5 (FAM19A5) ELISA Kit KB-E1714H

Regulatory Status:RUO

Alternative:FAM19A5;Family With Sequence Similarity 19, Member A5

Regulatory Status:

RUO

Alternative:

FAM19A5;Family With Sequence Similarity 19, Member A5

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

78-5000pg/mL

Sensitivity:

40pg/mL

Specificity:

This kit recognizes Human FAM19A5 in samples. No significant cross-reactivity or interference between Human FAM19A5 and analogues was observed.

Sample Type:

Tissue homogenates, cell lysates and other biological fluids

Research Area:

Signal Transduction


| FAM19A5


Chemokine-like protein TAFA-5 is a protein that is encoded by the TAFA5 gene.This gene is a member of the TAFA family which is composed of five highly homologous genes that encode small secreted Proteins. These Proteins contain conserved cysteine residues at fixed positions, and are distantly related to CCL3, a member of the CC-chemokine family. The TAFA Proteins are predominantly expressed in specific regions of the brain, and are postulated to function as brain-specific chemokines or neurokines, that act as regulators of immune and nervous cells.

 

| Typical data


As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.


  

Concentration (pg/mL)500025001250625312156780
OD2.361.340.80.480.340.260.220.09
Corrected OD2.271.250.710.390.250.170.13-

 





| Repeatability


Intra-assay Precision (Precision within an assay):CV% < 10%; Inter-assay Precision (Precision between assays):CV% < 10%

 

| Recovery


The recovery of Human FAM19A5 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.


 

Sample TypeRange(%)Average Recovery(%)
Tissue Homogenates(n=8)83-10196
Cell Culture Supernates(n=8)92-105101






 

| Linearity


Samples were spiked with high concentrations of Human FAM19A5 and diluted with Universal Diluent to produce samples with values within the range of the assay.


 



Tissue Homogenates(n=4)Cell Culture Supernates(n=4)
1 : 2Range (%)84-9586-96
Average (%)9193
1 : 4Range (%)89-10387-108
Average (%)9598









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