Human E3 ubiquitin-protein ligase UBR4 (UBR4) ELISA Kit KB-E0340H

Regulatory Status:

RUO

Alternative:

RBAF600; p600; ZUBR1; Zinc Finger,UBR1 Type 1; N-recognin-4; 600 kDa retinoblastoma protein-associated factor; Retinoblastoma-associated factor of 600 kDa;Ubiquitin Protein Ligase E3 Component N-Recognin 4

Detection Method:

ELISA

Assay Type:

Sandwich

Detection Range:

0.156-10ng/mL

Sensitivity:

0.071ng/mL

Specificity:

This kit recognizes Human UBR4 in samples. No significant cross-reactivity or interference between Human UBR4 and analogues was observed.

Sample Type:

Tissue homogenates and other biological fluids.

Research Area:

Epigenetics

| UBR4

E3 ubiquitin-protein ligase UBR4 is an enzyme that is encoded by the UBR4 gene.The protein encoded by this gene is an E3 ubiquitin-protein ligase that interacts with the retinoblastoma-associated protein in the nucleus and with calcium-bound calmodulin in the cytoplasm. The encoded protein appears to be a cytoskeletal component in the cytoplasm and part of the chromatin scaffold in the nucleus.Diseases associated with UBR4 include Alternating Hemiplegia Of Childhood 2 and Long Qt Syndrome. Among its related pathways are Class I MHC mediated antigen processing and presentation and Innate Immune System.
 

| Typical data

As the OD values of the standard curve may vary according to the conditions of the actual assay performance (e.g. operator, pipetting technique, wash technique or temperature effects), the operator should establish a standard curve for each test. Typical standard curve and data are provided below for reference only.

  

| Repeatability

Intra-assay Precision (Precision within an assay)：CV% < 10%; Inter-assay Precision (Precision between assays)：CV% < 10%
 

| Recovery

The recovery of Human UBR4 spiked at three different levels in samples throughout the range of the assay was evaluated in various matrices.

 

| Linearity

Samples were spiked with high concentrations of Human UBR4 and diluted with Universal Diluent to produce samples with values within the range of the assay.